The refuge was specifically created to protect the habitat of the Florida panther, of which fewer than 200 remain in the wild.
It is part of a larger network of public lands in Southwest Florida that constitutes the primary remaining range for the panther.
The landscape includes cypress sloughs, which are seasonal wetlands that act as the drainage system for the region.
The refuge conducts active forest management through prescribed fires to prevent hardwood encroachment into pine ecosystems.
The site is a major site for monitoring population health via radio-collared panthers managed by the Florida Fish and Wildlife Conservation Commission.
Visitors may occasionally spot alligator dens along the banks of the Scenic Lake Trail.
The Florida Panther National Wildlife Refuge serves as a critical habitat for the federally endangered Florida panther. Established in 1989, this 26,400-acre site protects a core breeding area within the Big Cypress Swamp ecosystem. The refuge contains a mosaic of pinelands, hardwood hammocks, and cypress sloughs designed to facilitate panther roaming and hunting. While panthers are the namesake, the area also supports wood storks, swallow-tailed kites, and various native orchids. Visitors primarily access the refuge via two designated walking trails, the Florida Panther Trail and the Scenic Lake Trail. The refuge functions as an ecological corridor linking the Big Cypress National Preserve to the north and the Fakahatchee Strand to the south. Management focuses on prescribed burning to maintain the natural landscape diversity necessary for endangered species survival.
The observation deck overlooking the Scenic Lake, which offers the best vantage point for water birds and potential alligator sightings.
Bring insect repellent and sun protection, as shade is inconsistent and mosquitoes are prevalent year-round.
Remain on marked trails at all times to avoid disturbing sensitive habitat and to protect yourself from uneven, muddy terrain.
Carry a physical map or downloaded GPS data, as cell service is unreliable throughout the refuge.
Do not attempt to hike off-trail, as the terrain is often swampy and poses a high risk of getting disoriented.
Trails are typically open daily from sunrise to sunset, but may close due to severe weather or seasonal flooding.
Stay on marked trails, pack out all trash, and maintain a quiet demeanor to increase chances of observing wildlife.